(+)-α-Tocopherol is a small molecule. Tocopherols are methyl-substituted hydroxychromans with a phytyl side chain. This product is semisynthetic. It is isolated from naturally occurring α-tocopherol found in vegetable oil. It is prepared using a by-product of the vegetable oil process called vegetable oil distillate (VOD) as its raw material. Soybean oil is used as a solvent in this process. α-Tocopherol is a powerful inhibitor of the proliferation of estrogen receptor positive and estrogen receptor negative human breast cancer cell lines in a dose dependent manner in vitro. Treatment at 15 µg/ml for 24 hours inhibited MDA-MB-435 cell proliferation by 71%. However, cells treated with this level of α-tocopherol exhibited reduced viability (81% vs. 96% for control cells). This product has been shown to interact with cytosolic Protein Kinase C in vascular smooth muscle cells. A review of various published research studies suggests that this product may help ward off heart attacks. α-Tocopherol is carried with LDLs and shields LDL from oxidation by free radicals. This protection leads to a decrease in LDL oxidation, which is a major cause in triggering artery stenosis. Artery blockage is due to immune cells engulfing oxidized LDL, which causes swelling and accumulation of fatty masses within the artery walls. Vitamin E may help prevent this. Isolation and analysis of tocopherols can be easily performed by a simple acetone extraction followed by HPLC.6 A C18 ODS2 column is packed with 3 µm particles and a methanol : water (99:1) mobile phase is used for isolation, resulting in detection and easy measurement of α-, δ-, and γ-tocopherol peaks. Fluorescence detection was performed with 290 nm excitation and 330 nm emission wavelengths. The equivalent of 1 mg of (+)-α-tocopherol is 1.49 International Units (IU). This oil contains approximately 400 mg of (+)-α-tocopherol per gram and is approximately 600 IU per gram.

Target	(+) alpha Tocopherol
Conjugation	Unconjugated
Storage	Store at room temperature.
Molecular Weight	430.7 Da
Molecular Formula	C29H50O2
CAS Number	59-02-9
Availability	Shipped within 5-10 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on (+) alpha Tocopherol

Isotype Control 同型对照 同型对照（Isotype Control），使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白，用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。 同型对照 同型对照的主要目的是确定一抗的结合是特异性的，而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体，与功能阻断抗体发挥同样的功能。 同型对照要与一抗的来源，Ig分型和标记完全一致。 如果一抗是多抗，可以用an normal serum（与一抗相同的正常血清） （must be the same species as primary antibody）。This control is easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来 调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat CD11b，clone OX-42 purified IgG，那么它的isotype 是mouse IgG2a， 所以可以用purified（纯化的） mouse IgG2a来做OX-42的同型对照（Isotype Control）。一般的的生物技术公司和国内的代理都有出售。 同型对照：是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类，若使用直接免疫荧光染色法，同型对照也应标记荧光色素，如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外，同型对照与MoAb所标记的荧光 色素、浓度、F：P比值（标记的荧光色素与免疫球蛋白分子的比值）应该相同为最佳，这对准确设定阴性与阳性细胞的界标有重要意义，切忌使用与MoAb不相 匹配的同型对照，最好为同一实验室、采用相同工艺或方法制备（如同一品牌）的产品。