Abbexa/14-3-3 Protein Beta/Alpha (YWHAB) Antibody/400 µl/abx033917-400 µl
商品编号：
abx033917-400µl
品牌：
Abbexa Ltd
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5" UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Research Articles on 14-3-3 Protein Beta/Alpha (YWHAB) Antibody
| Target | YWHAB |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | WB, IHC |
| Host | Rabbit |
| Recommended dilutions | Validated in IHC-P. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human YWHAB. |
| Isotype | IgG |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P31946 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K9K2, E1P616 |
| UniProt Entry Name | 1433B_HUMAN |
| NCBI Accession | NP_003395.1NP_647539.1 |
| KEGG | hsa:7529 |
| String | 9606.ENSP00000361930 |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
品牌介绍
Isotype Control
同型对照
同型对照（Isotype Control），使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白，用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。
同型对照
同型对照的主要目的是确定一抗的结合是特异性的，而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体，与功能阻断抗体发挥同样的功能。
同型对照要与一抗的来源，Ig分型和标记完全一致。
如果一抗是多抗，可以用an normal serum（与一抗相同的正常血清） （must be the same
species as primary antibody）。This control is
easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来 调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat
CD11b，clone OX-42 purified IgG，那么它的isotype 是mouse IgG2a， 所以可以用purified（纯化的） mouse IgG2a来做OX-42的同型对照（Isotype Control）。一般的的生物技术公司和国内的代理都有出售。
同型对照：是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类，若使用直接免疫荧光染色法，同型对照也应标记荧光色素，如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外，同型对照与MoAb所标记的荧光 色素、浓度、F：P比值（标记的荧光色素与免疫球蛋白分子的比值）应该相同为最佳，这对准确设定阴性与阳性细胞的界标有重要意义，切忌使用与MoAb不相 匹配的同型对照，最好为同一实验室、采用相同工艺或方法制备（如同一品牌）的产品。
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