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当前位置: 首页 > 产品中心 > peptide > Abbexa/Amyloid Beta 42 (Abeta42) ELISA Kit/5 × 96 tests/abx258778-5 × 96 tests
商品详细Abbexa/Amyloid Beta 42 (Abeta42) ELISA Kit/5 × 96 tests/abx258778-5 × 96 tests
Abbexa/Amyloid Beta 42 (Abeta42) ELISA Kit/5 × 96 tests/abx258778-5 × 96 tests
Abbexa/Amyloid Beta 42 (Abeta42) ELISA Kit/5 × 96 tests/abx258778-5 × 96 tests
商品编号: abx258778-5×96tests
品牌: Abbexa Ltd
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Amyloid Beta 42 (Abeta42) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Mouse, Rat Amyloid Beta 42 (Abeta42) concentrations in serum, plasma and other biological fluids.

Please note that this kit is also available as a CLIA Kit abx490591.

IntroductionBeta-Amyloid Protein 1-42 (Ab1-42) is a fragment of amyloid precursor protein (APP); other fragments exist with a length of 36-43 amino acids. These fragments can aggregate in the brain of Alzheimer’s disease patients and form misfolded “amyloid plaques” that induce other amyloid fragments to misfold and attach to the structure, much like a prion. Ab1-42 is the most abundant amyloid peptide found in these plaques. The function of amyloid protein in healthy humans is unknown, but its function may be related to enzyme regulation and detoxifying reactive oxygen species.
TargetAmyloid Beta 42 (Abeta42)
ReactivityMouse, Rat
Tested ApplicationsELISA
Recommended dilutionsOptimal dilutions/concentrations should be determined by the end user.
StorageShipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit"s manual.
ValidityThe validity for this kit is 6 months.
StabilityThe stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range12.35 pg/ml - 1000 pg/ml
Sensitivity< 6.2="">
Standard FormLyophilized
ELISA DetectionColorimetric
ELISA Type Competitive
ELISA DataQuantitative
Sample TypeSerum, plasma and other biological fluids.
Target TypeAntigen
Assay PrincipleThis kit is based on competitive enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the biotin-labelled Abeta42 and the unlabelled- Abeta42 on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient Abeta42 will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the Abeta42 amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of Abeta42 can be calculated.
Kit Components
  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Reagent Preparation
  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure
  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 50 µl of diluted standard into the standard wells.
  • 3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
  • 4) Aliquot 50 µl of diluted samples into the sample wells.
  • 5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Protocol
  • Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test.
  • 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
  • 2. Add 50 µL of each standard, control and sample into the appropriate wells. 3. Remove the cover and discard the liquid.
  • 4. Immediately aliquot 50 µl of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37°C.
  • 5.Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
  • 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min.
  • 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
  • 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
  • 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Results CalculationThis assay is competitive, therefore there is an inverse correlation between Abeta42 concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The Abeta42 concentration of the samples can be interpolated from the standard curve.
Assay PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Amyloid beta 42 (Abeta42) were were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Amyloid beta 42 (Abeta42) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) × 100
Intra-Assay: CV<>
Inter-Assay: CV<>
AvailabilityShipped within 5-7 working days.
NoteThis product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

Standard1000 pg/ml
Research Articles on Amyloid Beta 42 (Abeta42) ELISA Kit
品牌介绍
Isotype Control 同型对照 同型对照(Isotype Control),使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。 同型对照 同型对照的主要目的是确定一抗的结合是特异性的,而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体,与功能阻断抗体发挥同样的功能。 同型对照要与一抗的来源,Ig分型和标记完全一致。 如果一抗是多抗,可以用an normal serum(与一抗相同的正常血清) (must be the same species as primary antibody)。This control is easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同,应选用相同来源的未标记单抗作为同型对照来 调整背景染色。举个例子:比如检测一抗为单抗的mouse anti rat CD11b,clone OX-42 purified IgG,那么它的isotype 是mouse IgG2a, 所以可以用purified(纯化的) mouse IgG2a来做OX-42的同型对照(Isotype Control)。一般的的生物技术公司和国内的代理都有出售。 同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接免疫荧光染色法,同型对照也应标记荧光色素,如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外,同型对照与MoAb所标记的荧光 色素、浓度、F:P比值(标记的荧光色素与免疫球蛋白分子的比值)应该相同为最佳,这对准确设定阴性与阳性细胞的界标有重要意义,切忌使用与MoAb不相 匹配的同型对照,最好为同一实验室、采用相同工艺或方法制备(如同一品牌)的产品。