Abbexa/抗体对支持试剂盒（竞争法）/10×96试验/abx098959-10×96试验
商品编号：
abx098959-10×96tests
品牌：
Abbexa Ltd
市场价：
¥5220.00
美元价：
5220.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Antibody Pair Support Kit (Competitive Method) contains reagents for use with an antibody pair for a Competitive ELISA or CLIA.
Kit contents:
- Coating Buffer: 1 vial, lyophilized powder
- Blocking Buffer: 1 vial, lyophilized powder
- Standard Diluent: 1 vial, lyophilized powder
- Biotin-Conjugated Competitor Diluent: 1 vial, lyophilized powder
- Streptavidin-HRP Diluent: 1 vial, lyophilized powder
- Wash Buffer: 2 vials, lyophilized powder
- Streptavidin-HRP: 1 vial, 1.2 ml
- TMB Substrate Solution: 2 vials, 48 ml each
- Stop Solution: 2 vials, 30 ml each
- Coating Buffer: Reconstitute in 220 ml distilled water.
- Blocking Buffer: Reconstitute in 240 ml distilled water.
- Standard Diluent: Reconstitute in 160 ml distilled water.
- Biotin-Conjugated Competitor Diluent: Reconstitute in 120 ml distilled water.
- Streptavidin-HRP Diluent: Reconstitute in 120 ml distilled water.
- Wash Buffer: Reconstitute 1 vial in 2000 ml distilled water.
| Reactivity | General (All species) |
| Tested Applications | ELISA |
| Recommended dilutions | Streptavidin-HRP should be diluted 1/100 using Streptavidin-HRP Diluent. |
| Reconstitution | Reconstitute and prepare working solutions before carrying out the assay. All working solutions should be colourless. |
| Storage | Store Streptavidin-HRP at -20 °C. All other components can be stored at 4 °C for up to 6 months. Reconstituted and working (appropriately diluted) solutions should be used immediately but can be stored at 4 °C for up to one week. Avoid repeated freeze/thaw cycles. It is recommended that solutions are made fresh before the experiment. |
| Buffer | The Stop Solution contains 0.1% sulphuric acid. |
| Directions for use | Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. |
品牌介绍
Isotype Control
同型对照
同型对照（Isotype Control），使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白，用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。
同型对照
同型对照的主要目的是确定一抗的结合是特异性的，而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体，与功能阻断抗体发挥同样的功能。
同型对照要与一抗的来源，Ig分型和标记完全一致。
如果一抗是多抗，可以用an normal serum（与一抗相同的正常血清） （must be the same
species as primary antibody）。This control is
easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来 调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat
CD11b，clone OX-42 purified IgG，那么它的isotype 是mouse IgG2a， 所以可以用purified（纯化的） mouse IgG2a来做OX-42的同型对照（Isotype Control）。一般的的生物技术公司和国内的代理都有出售。
同型对照：是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类，若使用直接免疫荧光染色法，同型对照也应标记荧光色素，如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外，同型对照与MoAb所标记的荧光 色素、浓度、F：P比值（标记的荧光色素与免疫球蛋白分子的比值）应该相同为最佳，这对准确设定阴性与阳性细胞的界标有重要意义，切忌使用与MoAb不相 匹配的同型对照，最好为同一实验室、采用相同工艺或方法制备（如同一品牌）的产品。
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