Abbexa/11β-羟基类固醇脱氢酶2型(HSD11b2)抗体/1 mg/abx130057-1 mg
商品编号:
abx130057-1mg
品牌:
Abbexa Ltd
市场价:
¥26100.00
美元价:
26100.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
11-Beta-Hydroxysteroid Dehydrogenase Type 2 Antibody is a Rabbit Polyclonal against 11-Beta-Hydroxysteroid Dehydrogenase Type 2.
Research Articles on 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) Antibody
Target | 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) |
Clonality | Polyclonal |
Reactivity | Rat |
Tested Applications | WB, IHC, IF/ICC, IP |
Host | Rabbit |
Recommended dilutions | WB: 0.2-2 µg/ml, IHC: 5-20 µg/ml, IF/ICC: 5-20 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | HSD11b2 (Leu58-Pro393) |
Isotype | IgG |
Form | Liquid |
Purification | Purified by antigen-specific affinity chromatography, followed by Protein A affinity chromatography. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P50233 (UniProt, ExPASy) |
UniProt Entry Name | DHI2_RAT |
String | 10116.ENSRNOP00000023130 |
Buffer | PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol. |
Specificity | The antibody is a rabbit polyclonal antibody raised against HSD11b2. It has been selected for its ability to recognize HSD11b2 in immunohistochemical staining and western blotting. |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. |
品牌介绍
Isotype Control
同型对照
同型对照(Isotype Control),使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白,用于消除由于抗体非特异性结合到细胞表面而产生的背景染色。
同型对照
同型对照的主要目的是确定一抗的结合是特异性的,而不是非特异性的Fc受体或与其他蛋白的相互作用。还可以用来竞争性的结合抗体,与功能阻断抗体发挥同样的功能。
同型对照要与一抗的来源,Ig分型和标记完全一致。
如果一抗是多抗,可以用an normal serum(与一抗相同的正常血清) (must be the same
species as primary antibody)。This control is
easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同,应选用相同来源的未标记单抗作为同型对照来 调整背景染色。举个例子:比如检测一抗为单抗的mouse anti rat
CD11b,clone OX-42 purified IgG,那么它的isotype 是mouse IgG2a, 所以可以用purified(纯化的) mouse IgG2a来做OX-42的同型对照(Isotype Control)。一般的的生物技术公司和国内的代理都有出售。
同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接免疫荧光染色法,同型对照也应标记荧光色素,如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外,同型对照与MoAb所标记的荧光 色素、浓度、F:P比值(标记的荧光色素与免疫球蛋白分子的比值)应该相同为最佳,这对准确设定阴性与阳性细胞的界标有重要意义,切忌使用与MoAb不相 匹配的同型对照,最好为同一实验室、采用相同工艺或方法制备(如同一品牌)的产品。
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